Staining for BromodeoxyUridine (BrdU) Immunohistochemistry
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چکیده
منابع مشابه
Immunohistochemistry and BrdU-LTR assay
The procedures for bone and bone marrow section preparation, immunostaining conditions and antibodies are described in Supplementary Methods. The procedure for BrdU pulse labeling, LTR and subsequent detection has been reported. The mice were fed BrdU (0.8 mg ml in water) for 10 days, during which time 40% of LT-HSCs would divide at least once. Seventy days after BrdU labelling, sections were s...
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INTRODUCTIONThe number of cells traversing the cell cycle and the rate of progression through it provide important indices of cell growth and tumorigenicity. S-phase cells can also be identified by their high content of DNA polymerase and proliferating cell nuclear antigen, a component of the leading-strand polymerase. Although both these markers can be detected rapidly and conveniently using t...
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A direct Giemsa technique is described for the selective staining of Brd-U-substituted chromatin. It can advantageously be used for sister-chromatid exchange analysis and for the detailed cytological study of the mouse kinetochores. Presumptive inversions within the centromeric region of chromosomes 4 and 18 in the C57Black/6J mouse are described.
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Microbes are major agents mediating the degradation of numerous dissolved organic carbon (DOC) substrates in aquatic environments. However, identification of bacterial taxa that transform specific pools of DOC in nature poses a technical challenge. Here we describe an approach that couples bromodeoxyuridine (BrdU) incorporation, fluorescence activated cell sorting (FACS), and 16S rRNA gene-base...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 1999
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500063951